• 来稿:陈琳   北京市虹天济神经科学研究院

  • Mol Ther. 2004 Nov;10(5):844-54.  

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  • Vascular endothelial growth factor stimulates skeletal  muscle regeneration in vivo

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  • Arsic N, Zacchigna S, Zentilin L, Ramirez-Correa G,  Pattarini L, Salvi A, Sinagra G, Giacca  M.

  • Molecular Medicine Laboratory, International Center for Genetic Engineering and Biotechnology,  Padriciano 99, 34012 Trieste, Italy.

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  • Vascular endothelial growth factor  (VEGF) is a major regulator of blood vessel formation during development and in  the adult organism. Recent evidence indicates that this factor also plays an  important role in sustaining the proliferation and differentiation of different  cell types, including progenitor cells of different tissues, including bone  marrow, bone, and the central nervous system. Here we show that the delivery of  the 165-aa isoform of VEGF-A cDNA using an adeno-associated virus (AAV) vector  exerts a powerful effect on skeletal muscle regeneration in vivo. Following  ischemia-, glycerol-, or cardiotoxin-induced damage in mouse skeletal muscle,  the delivery of AAV-VEGF markedly improved muscle fiber reconstitution with a  dose-dependent effect. The expression of both VEGF receptor-1 (VEGFR-1) and  VEGFR-2 was upregulated both in the satellite cells of the damaged muscles and  during myotube formation in vitro; the VEGF effect was mediated by the VEGFR-2,  since the transfer of PlGF, a VEGF family member interacting with the VEGFR-1,  was ineffective. These results are consistent with the observation that VEGF  promotes the growth of myogenic fibers and protects the myogenic cells from  apoptosis in vitro and prompt a therapeutic use for VEGF gene transfer in a  variety of muscular disorders.

 

 

中枢神经系统中的神经元再生
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血管内皮生长因子体内刺激骨骼肌再生